Structural investigation of the antibiotic sporaviridin. XIV. Isolation of components of intact sporaviridin.

Six components of sporaviridin (SVD) were successfully isolated by HPLC using methano-1 M ammonium chloride as the mobile phase. Each component possesses expectedly antimicrobial activity. To ensure the structural relationship between the intact SVD and N-acetylated sporaviridins (N-Ac-SVD), they were converted to the corresponding of N-acetates by acetylation in methanol, whose structures had already been determined as glycosides consisting of a 34-membered macrocyclic lactone, viridopentaoses, D-glucose and N-acetylvancosamine.